Revision 7
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IF-F

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Product Information

Storage

Store at room temperature. Protect from light. This product is stable for 12 months when stored at room temperature.

Safety Information: This product contains Dimethylformamide (DMF). DMF is rapidly absorbed through skin and lungs. It may cause harm to the unborn child. Avoid exposure during pregnancy. If exposed or concerned, get medical advice. See Safety Data Sheet (SDS).

Wear personal protective equipment. Avoid contact with skin. Do not handle until all safety precautions have been read and understood.

Specificity / Sensitivity

In addition to significantly reducing autofluorescence, TrueBlack® Lipofuscin Autofluorescence Quencher may cause a decrease in signal with some antibodies by immunofluorescence. We do not find this decrease to be significant enough to limit interpretation of data.

Product Description

TrueBlack® Lipofuscin Autofluorescence Quencher is specifically formulated to reduce autofluorescence from lipofuscin, a material that can accumulate in aged human and animal tissues. This product can also reduce general background fluorescence, as well as autofluorescence from other sources such as collagen, elastin, and red blood cells.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Revision 7
#92401

TrueBlack® Lipofuscin Autofluorescence Quencher

Immunofluorescence Image 1: TrueBlack® Lipofuscin Autofluorescence Quencher Expand Image
使用 AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #82941(绿色)、α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #60839(红色)和 DAPI #4083(蓝色)对未经处理(下图)或经过 TrueBlack® Lipofuscin Autofluorescence Quencher(上图)处理的固定冷冻的小鼠肾脏进行共聚焦免疫荧光分析。
Immunofluorescence Image 2: TrueBlack® Lipofuscin Autofluorescence Quencher Expand Image
使用 ProLong® Gold Antifade Reagent with DAPI #8961(蓝色)对未经处理(左图)或经过 TrueBlack® 脂褐素自发荧光淬灭剂(右图)处理且来自阿尔茨海默病淀粉样蛋白小鼠模型的固定冷冻的小鼠中脑进行共聚焦免疫荧光分析。绿色信号显示脂褐素自发荧光。
Immunofluorescence Image 3: TrueBlack® Lipofuscin Autofluorescence Quencher Expand Image
使用 Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617(绿色)和 DAPI #4083(蓝色)对未经处理(左图)或经过 TrueBlack® Lipofuscin Autofluorescence Quencher处理的固定冷冻的小鼠中脑进行共聚焦免疫荧光分析。白色箭头指向自发荧光。