SignalStain^® Radiant Yellow Peroxidase Substrate Kit

1. To prepare the Radiant Yellow/HRP chromogen-substrate working solution, add 1 drop SignalStain^® Radiant Yellow Chromogen Concentrate to 1 mL SignalStain^® Radiant Yellow Diluent. Mix well before use. Working solutions are stable for up to 1 day when stored at 4ºC. For optimal results, prepare fresh reagent.

2. After incubation with SignalStain^® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 8-20 minutes generally provides an acceptable staining intensity.

3. Wash slides for 5 minutes in water.

4. Counterstain, if desired. Radiant Yellow is compatible with Hematoxylin #14166.

5. After rinsing, slides should be air dried and mounted using SignalStain^® Aqueous Mounting Medium #27290. Radiant Yellow is not compatible with alcohol.

Alternatively, for best color preservation and long-term storage, SignalStain^® Mounting Prep Solution #50815 can be used after counterstaining to enable mounting with non-aqueous mounting medium, SignalStain^® Mounting Medium #14177.

Appropriate personal protective equipment should be worn when handling Radiant Yellow. Dispose of in accordance with local regulations.

Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 6 months upon receipt if the reagents are not combined.

The SignalStain^® Radiant Yellow Peroxidase Substrate Kit contains all of the necessary reagents to prepare a working solution of Radiant Yellow for staining tissue sections. The Radiant Yellow working solution is compatible with peroxidase (HRP) detection systems such as the SignalStain^® Boost IHC Detection Reagents (HRP, Rabbit #8114, HRP, Mouse #8125, HRP, Rat #72838, and HRP, Goat #63707), yielding a yellow reaction product that can provide color contrast in pigmented tissues such as melanoma.