MitoTracker® Green FM (#9074) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IF-IC, FC-FP

Product Information

Product Usage Information

Each vial of MitoTracker^® Green FM contains 50 µg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 74.4 µl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into normal growth media at a concentration between 100-400 nM and incubating between 15-30 min at 37ºC. After incubation, the cells must be imaged live; DO NOT FIX!

To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.

Storage

Store lyophilized solid at -20°C dessicated and protected from light. In lyophilized form, this reagent is stable for 12 months. Once reconstituted in DMSO, the solution should be stored at -20°C, protected from light, and must be used within 2 weeks. Avoid freeze-thaw cycles.

Product Description

MitoTracker^® Green FM is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 490 nm, Emission: 516 nm, Molecular Weight: 671.88 g/mol

Background

MitoTracker^® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker^® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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