Product Information
Product Usage Information
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Product Description
| Molecular Weight | 652.2 g/mol |
| Purity | >98% |
| Molecular Formula | C25H27Cl4IN4 |
| CAS | 3520-43-2 |
| Solubility | Soluble in DMSO at 15 mg/mL or water at 50 mg/mL. |
Background
JC-1 is a membrane-permeable cyanine dye used to measure mitochondrial membrane potential. JC-1 is a dual-emission, green-fluorescent dye (emission maximum at approximately 520 nm) at low membrane potential and forms red-fluorescence (emission maximum at 596 nm) at higher membrane potentials (1). Monomeric JC-1 is the predominant form in cells exhibiting lower mitochondrial membrane potential, while aggregates of JC-1 (“J-aggregates”) are found in cells with higher mitochondrial membrane potential (2). JC-1 can be used to detect cells undergoing apoptosis, with healthy cells exhibiting red-fluorescence and cytoplasmic JC-1 exhibiting green-fluorescence in apoptotic cells (3). JC-1 is also a substrate of the multidrug transporter P-glycoprotein (P-gp, MDR1), an ATP-binding cassette transporter protein associated with development of multidrug resistance. Transport of JC-1 by P-gp from cells can reduce cellular JC-1 levels, creating a false indication of decreased mitochondrial membrane potential (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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