0.5 M EDTA, pH 8.0 (#7011) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Product Usage Information

This product is supplied ready to use.

Storage

Store at 4°C. This product is stable for 24 months.

Product Description

EDTA (Ethylenediaminetetraacetic acid) is a common laboratory chelating agent of divalent cations, such as Ca²⁺ and Mg²⁺. Ultrapure 0.5 M EDTA, pH 8.0 from Cell Signaling Technology contains no detectable DNase, RNase, or protease activity. The convenient 1 ml vials reduce the likelihood of contamination that can occur with larger volume containers. It is suitable for use in molecular biology or protein biochemistry applications that require the chelation of divalent metal cations.

This product is used in our SimpleChip^® chromatin immunoprecipitation (ChIP) assays to stop the metal-dependant enzymatic digestion of cross-linked DNA by micrococcal nuclease once the reaction is complete. It can be added to cell lysis buffers for use as a metalloprotease inhibitor. Working concentrations are typically 1-5 mM in this application.

Molecular Formula	C₁₀H₁₄N₂O₈Na₂•2H₂O

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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