Protein L (PE Conjugate) (#58036) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

FC-L

Product Information

Product Usage Information

Recommended Dilution: 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot. Protect from light. Do not freeze.

Specificity / Sensitivity

Protein L (PE Conjugate) is recombinantly derived and genetically engineered such as to remove the cell wall-, cell membrane-, and albumin-binding regions of Protein L to enhance binding to immunoglobulins. Conjugation to PE makes this product ideally suited for direct flow cytometric analysis of cells expressing κ light chain-containing immunoglobulins as well as immunoglobulin fragments that contain κ light chains, such as scFvs.

Species Reactivity:

Human, Mouse, Rat, Pig

Product Description

This Cell Signaling Technology product is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in human cells.

Background

Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus (1). Unlike Protein A and Protein G that differentially bind to the Fc region of immunoglobulin isotype heavy chains, Protein L contains Ig binding domains that interact with the κ light chain variable domain without disrupting paratope function (2-4). This attribute not only facilitates binding to a broader range of immunoglobulin classes and subclasses than either Protein A or Protein G but also allows for binding to Fab fragments and single chain variable fragments (scFvs) that contain κ light chain sequences. Protein L does, however, display specificity with respect to κ light chain subtypes. In human, Protein L binds to VκI, VκIII, and VκIV subtypes whereas binding is restricted to VκI in mice (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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