Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IF-IC, FC-FP

REACTIVITY:

H R Mk

SENSITIVITY:

MW (kDa):

SOURCE:

Rabbit

Product Information

Product Usage Information

Application Dilution
Immunofluorescence (Immunocytochemistry) 1:1000
Flow Cytometry (Fixed/Permeabilized) 1:200

Storage

Supplied in PBS, 100 ug/ml BSA and 0.1% sodium azide. Store at 4 C. Do not aliquot the antibody.

Specificity / Sensitivity

Myc-Tag Antibody (Fluorescein Conjugate) detects Myc-tagged recombinant proteins and is a useful tool for subcellular protein localization. The antibody is conjugated to fluorescein via amino-terminal amines and the epsilon amino groups on lysine. Fluorescein absorbs visible blue light at wavelength 490 nm and emits visible green light at 520 nm.

Species Reactivity:

Human, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

  1. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Revision 1
#2277

Myc-Tag Antibody (Fluorescein Conjugate)

Immunofluorescence Image 1: Myc-Tag Antibody (Fluorescein Conjugate) Expand Image
使用 Myc-Tag Antibody (Fluorescein Conjugate) 对表达带有 c-Myc 标签的蛋白的 SH-SY5Y 神经母细胞瘤细胞 (D) 或对照细胞 (B) 进行免疫细胞化学染色。图像 A 和 C 分别为 B 和 D 染色细胞的光显微照片。
No image available