| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (Alexa Fluor® 647 Conjugate) | 13148 | 40 µl | 42, 44 kDa | Rabbit IgG |
| Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 647 Conjugate) | 4552 | 40 µl | 40 kDa | Mouse IgG1 |
| Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor® 647 Conjugate) | 9257 | 40 µl | 46 Phospho-JNK1. 54 Phospho-JNK2/3. kDa | Mouse IgG1 |
| p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 647 Conjugate) | 5376 | 40 µl | Rabbit IgG | |
| Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) | 4843 | 40 µl | Mouse IgG1 | |
| Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) | 2985 | 40 µl | Rabbit IgG |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The MAPK Family Alexa Fluor® 647 Conjugated Antibody Sampler Kit provides an economical means to study the activation status of members of the MAPK family of proteins without the need for a fluorescent secondary antibody.
Storage
Background
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (5-7), and research investigators consider it an important target in the diagnosis and treatment of cancer (8). The stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (9).
- Rouse, J. et al. (1994) Cell 78, 1027-37.
- Han, J. et al. (1994) Science 265, 808-11.
- Lee, J.C. et al. (1994) Nature 372, 739-46.
- Freshney, N.W. et al. (1994) Cell 78, 1039-49.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
Background References
Trademarks and Patents
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