Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

FC-FP

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q9UJ71

Entrez-Gene Id:

50489

Product Information

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Langerin (D9H7R) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Langerin protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val14 of human langerin protein.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Langerin (D9H7R) XP® Rabbit mAb #13650.

Background

Langerin (CD207) is a C-type lectin receptor whose expression is restricted mainly to dendritic cells in the skin including Langerhans cells in the epidermis and langerin+/CD103+ dermal dendritic cells (1-4). Langerin is found on the cell surface and within rod-shaped organelles called Birbeck granules, and its expression is required for the formation of Birbeck granules (1,5). Langerin recognizes carbohydrate motifs on the surface of pathogens, resulting in endocytosis of the pathogen into Birbeck granules, degradation of the pathogen, and antigen presentation to T cells (6-8).

  1. Valladeau, J. et al. (2000) Immunity 12, 71-81.
  2. Poulin, L.F. et al. (2007) J Exp Med 204, 3119-31.
  3. Ginhoux, F. et al. (2007) J Exp Med 204, 3133-46.
  4. Bursch, L.S. et al. (2007) J Exp Med 204, 3147-56.
  5. Kissenpfennig, A. et al. (2005) Mol Cell Biol 25, 88-99.
  6. Stambach, N.S. and Taylor, M.E. (2003) Glycobiology 13, 401-10.
  7. Idoyaga, J. et al. (2008) J Immunol 180, 3647-50.
  8. Igyártó, B.Z. et al. (2011) Immunity 35, 260-72.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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Revision 1
#90662

Langerin (D9H7R) XP® Rabbit mAb (PE Conjugate)

Flow Cytometry Image 1: Langerin (D9H7R) XP® Rabbit mAb (PE Conjugate) Expand Image
使用 Langerin (D9H7R) XP® Rabbit mAb (PE Conjugate) 对未分化(蓝色)或经 Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (10ng/ml)、Human Transforming Growth Factor β1 (hTGF-β1) #8915 (10ng/ml) 和 Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5ng/ml) 处理 10 天后分化(绿色)成类朗格汉斯细胞的 MUTZ-3 细胞进行流式细胞分析。