WB
H
Endogenous
35, 85
Rabbit IgG
#P14210
3082
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human HGF protein.
Product Description
MW (kDa) | 35, 85 |
Background
The Hepatocyte Growth Factor (HGF, also known as Scatter Factor) was initially discovered as a mitogenic cytokine that induced hepatocyte replication and proliferation (1-3). HGF is produced by stromal cells where it is processed by extracellular serine proteases into a heterodimer consisting of alpha and beta subunits (4). Through activation of its receptor, cMET, HGF has a wide range of effects beyond hepatocytes that includes angiogenesis, epithelial cell proliferation and morphogenesis, and tissue protection and regeneration (5). The HGF-cMET axis has been associated with several diseases, including cancer, where HGF has been shown to promote invasion, metastasis, and drug resistance (6,7). These research studies suggest that HGF is a potential diagnostic and therapeutic target.
- Nakamura, T. et al. (1984) Biochem Biophys Res Commun 122, 1450-9.
- Russell, W.E. et al. (1984) J Cell Physiol 119, 183-92.
- Gohda, E. et al. (1988) J Clin Invest 81, 414-9.
- Kataoka, H. et al. Cancer Metastasis Rev 22, 223-36.
- Nakamura, T. and Mizuno, S. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86, 588-610.
- Matsumoto, K. and Nakamura, T. (2006) Int J Cancer 119, 477-83.
- Yano, S. et al. (2008) Cancer Res 68, 9479-87.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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