Revision 5
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IF-F, FC-FP, FC-L

REACTIVITY:

M

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rat IgG2a kappa

UniProt ID:

#P18337

Entrez-Gene Id:

20343

Product Information

Product Usage Information

For optimal flow cytometry results, we recommend 0.25μg of antibody per test.

Application Dilution
Immunofluorescence (Frozen) 1:400 - 1:800
Flow Cytometry (Fixed/Permeabilized) 1:200
Flow Cytometry (Live) 1:200

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

CD62L/L-Selectin (MEL-14) Rat mAb (FITC Conjugate) recognizes endogenous levels of total mouse CD62L protein.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Product Description

This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells.

Background

L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).

  1. Gallatin, W.M. et al. Nature 304, 30-4.
  2. Tedder, T.F. et al. (1989) J Exp Med 170, 123-33.
  3. Tedder, T.F. et al. (1990) J Immunol 144, 532-40.
  4. Berg, E.L. et al. (1991) J Cell Biol 114, 343-9.
  5. Finger, E.B. et al. (1996) Nature 379, 266-9.
  6. Sallusto, F. et al. (1999) Nature 401, 708-12.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-F: Immunofluorescence (Frozen) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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