Revision 1
Cell Signaling Technology

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Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 488 Conjugate) 9603 40 µl Rabbit IgG
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) 9148 40 µl 89 kDa Rabbit IgG
Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) 11882 40 µl Rabbit IgG
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) 2975 40 µl Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to study apoptosis and proliferation status using known markers without the need for a fluorescent secondary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). PARP is involved in DNA repair in response to environmental stress (2). Ki-67 is a nuclear nonhistone protein that is universally expressed among proliferating cells and absent in quiescent cells (3,4). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-8.
  3. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  4. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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