Background
PX-866 is a potent derivative of wortmannin that irreversibly inhibits PI3 kinase (1). Research studies demonstrate that PX-866 elicits a more sustained inhibition of PI3K than wortmannin. While both chemicals inhibit Akt phosphorylation similarly at short time periods (1-2 hr), PX-866 also demonstrates inhibitory effects for extended time periods (24 hr) at lower doses than wortmannin. This difference may be attributed to wortmannin’s short half-life of 10 minutes and the reduced cytotoxicity of PX-866 (2). PX-866 inhibits the PI3K pathway through down regulation of cyclin D1 protein, which is responsible for regulating cell cycle progression and autophagy induction (3). Additional research shows that PX-866 inhibits cell migration in cancer cell lines (2).
Molecular Formula
C29H35NO8
Molecular Weight
525.59 g/mol
Purity
>99%
CAS
502632-66-8
Solubility
Soluble in DMSO and EtOH at 200mg/ml.
Storage
Directions for Use
PX-866 is supplied as a lyophilized powder. For a 10mM stock, reconstitute the 1 mg in 190.26 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10 nM-1000 nM for 1-24 hours.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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