Mitochondrial Fusion Promoter M1 (#55199) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Background

Mitochondrial Fusion Promoter M1 is a hydrazone compound that promotes mitochondrial fusion in fragmented mitochondria (1). In a study of human induced pluripotent stem cells (iPSCs), M1 fused fragmented mitochondria and promoted the differentiation of iPSCs into an early mesodermal cardiac lineage (2). Pancreatic β cells treated with soluble cholesterol triggers increased total cholesterol accumulation, diminished cellular respiration, and compromised glucose-stimulated insulin secretion. Treatment of these cells with M1 prevents cholesterol-mediated suppression of cellular respiration and increases glucose-stimulated insulin secretion (3). M1 is also seen to protect against brain damage in rats with induced cardiac ischemia/reperfusion (I/R) injury (4) and against diabetic cardiomyopathy in a rat model of diabetes (5). Treatment of donor microvascular endothelial cells with M1 and the fission inhibitor Mdivi1 promotes mitochondrial fusion and reduces recipient T cell responses, which may lead to improved cardiac transplant survival (6).

Molecular Formula

C₁₄H₁₀Cl₄N₂O

Molecular Weight

364.1 g/mol

Purity

>98%

CAS

219315-22-7

Solubility

Soluble in DMSO at 40 mg/mL or ethanol at 6 mg/mL.

Storage

Store lyophilized at room temperature, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 2 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Directions for Use

Mitochondrial Fusion Promoter M1 is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 0.92 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Background References

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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