Mithramycin A (#61471) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Background

Mithramycin A is an antibiotic that binds the minor groove in GC-rich regions of DNA to inhibit transcription factor-DNA binding and prevent transcriptional activation (1). Mithramycin A is a specific inhibitor of specificity protein 1 (SP1), a ubiquitously expressed transcription factor that plays a role in control of cell growth and differentiation, apoptosis, and the immune response (2). Inhibition of SP1 by Mithramycin A leads to suppression of downstream targets, including proto-oncogenes ha-RAS12 and c-Myc13, anti-apoptotic genes survivin14 and XIAP15, and the pro-angiogenic gene VEGF. Mithramycin A inhibition of SP1 suppresses anti-apoptotic gene expression and induces caspase-dependent apoptosis (3). Several studies demonstrated that nanomolar concentrations of Mithramycin A could suppress tumor growth with no apparent effect on normal cell division. Mithramycin A was used therapeutically to treat testicular cancer and myeloid leukemia. Additional work suggests a possible role of Mithramycin A in treating cervical and colorectal cancer (4).

Molecular Formula

C₅₂H₇₆O₂₄

Molecular Weight

1085.2 g/mol

Purity

>98%

CAS

18378-89-7

Solubility

Soluble in DMSO at 20 mg/mL or ethanol at 10 mg/mL.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Directions for Use

Mithramycin A is supplied as a lyophilized powder. For a 1 mM stock, reconstitute 1 mg of powder in 0.92 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Background References

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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