Chromomycin A3 (#79929) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Product Usage Information

Chromomycin A3 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute 5 mg of powder in 0.84 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight	1183.3 g/mol
Purity	>98%
Molecular Formula	C₅₇H₈₂O₂₆
CAS	7059-24-7
Solubility	Soluble in DMSO at 20 mg/mL.

Background

Chromomycin A3 is an antibiotic that binds to the GC-rich sequence of DNA in the presence of divalent cations, inhibiting DNA replication and transcription (1). It is also used as a fluorescent DNA stain, and is particularly useful in staining human sperm chromatin in fertility and protamine deficiency studies (2). An analog of Mithramycin A, Chromomycin A3 displays many of the same DNA-binding and transcriptional regulation properties. For example, Chromomycin A3 suppresses a similar set of specificity protein 1 (SP1)-related anti-apoptotic proteins as Mithramycin A, and effectively inhibits cell proliferation more strongly than Mithramycin A. In a comparative treatment of cervical cancer cells, Chromomycin A3 displayed an IC₅₀ that was 4-10 times higher than the same cells treated with Mithramycin A. In several clinical studies, Chromomycin A3 exhibited anti-cancer effects in individuals with breast cancer, Hodgkin's disease, lung adenocarcinoma, melanoma, and rhabdomyosarcoma; however, there were also inconsistent treatment results and some harmful side effects (3).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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