Revision 2

#95580

Store at -20C

Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006)
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
SCA-ITS3

Reactivity:
H M

Sensitivity:
Endogenous

Source/Isotype:
Rabbit IgG

UniProt ID:
#P16220

Entrez-Gene Id:
1385

Storage

Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006) detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

SCA-ITS3: Single Cell Analysis - InTraSeq™ 3'

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

10x Genomics, 10x, Feature Barcode, and Chromium are the trademarks or registered trademarks of 10x Genomics, Inc.

InTraSeq is a trademark of Cell Signaling Technology, Inc.

Subject to patents licensed from 10x Genomics, Inc. for use with single-cell (i.e., Chromium) 10x products.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

#95580

Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006)

Cell Signaling Technology Logo
Cultured human peripheral blood mononuclear cells (PBMCs) treated with Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255 (30 min; Stimulated, left panels) or treated with Cell Stimulation Cocktail (without Protein Transport Inhibitors (500X) #29255 (30 min) then subsequently treated with Lambda Protein Phosphatase (40 min, 30°C, Stimulated + Phosphatase; right panels) were processed with the InTraSeq™ 3’ protocol. The T cell population was then selected and analyzed. The FeaturePlots in the top figures display the Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006) expression on the Uniform Manifold Approximation and Projection (UMAP). The FeaturePlots in the bottom figures display the CREB (48H2) Rabbit mAb (InTraSeq™ 3' Conjugate 3008) #41320 expression on the UMAP. To generate similar plots, use the FeaturePlot command template from the Seurat computational package: FeaturePlot(your_object, features = your_protein, max.cutoff = "q95", min.cutoff = "q5", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))
SCA Image 1: Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq<sup>™</sup> 3' Conjugate 3006)
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.