Revision 7
#8240
Store at -20C
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IF-IC, FC-FP, ChIP, ChIP-seq, C&R, C&T
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
23
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q96QV6
Entrez-Gene Id:
221613
Product Usage Information
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:3200 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #45772.
Specificity/Sensitivity
Source / Purification
Background
Background References
- Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
- Liu, F. and Walters, K.J. (2010) Trends Biochem Sci 35, 352-60.
- Wang, H. et al. (2004) Nature 431, 873-8.
- Cao, R. et al. (2005) Mol Cell 20, 845-54.
- de Napoles, M. et al. (2004) Dev Cell 7, 663-76.
- Fang, J. et al. (2004) J Biol Chem 279, 52812-5.
- Ginjala, V. et al. (2011) Mol Cell Biol 31, 1972-82.
- Bergink, S. et al. (2006) Genes Dev 20, 1343-52.
- Marteijn, J.A. et al. (2009) J Cell Biol 186, 835-47.
- Wu, J. et al. (2009) Mol Cell Biol 29, 849-60.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq C&R: CUT&RUN C&T: CUT&Tag
Cross-Reactivity Key
H: Human M: Mouse R: Rat Mk: Monkey
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
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Revision 7
Revision 7
Revision 7
Revision 7
