Revision 5
#8059
Store at +4C
SignalStain® DAB Substrate Kit
Petite 1 Kit
(120 slides)
Small 1 Kit
(1200 slides)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Quantity (Petite) | Quantity (Small) |
|---|---|---|---|
| SignalStain® DAB Diluent | 11724 | 1 x 12 ml | 1 x 120 ml |
| SignalStain® DAB Chromogen Concentrate | 11725 | 1 x 360 µl | 1 x 3.6 ml |
Description
Storage
Directions for Use
To prepare the DAB working solution, add 1 drop (30 μl) SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent and mix well before use. Working solutions are stable for up to 14 days when stored at 4ºC or up to 5 days when stored at room temperature (approximately 25ºC). While the solution may change color over that time period, it will have no effect on the quality of the staining.
*Please note that when using 8059P, a dropper for the chromogen concentrate is not included. Use a pipet to add 30 μl of SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent.
Important: DAB is a suspected carcinogen. Good laboratory practices should be followed, and appropriate protective equipment should be used when handling DAB, including gloves, protective eyeware, and a lab coat. Dispose in accordance with local regulations.
After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 1-10 minutes generally provides an acceptable staining intensity.
Wash slides in water for 5 minutes.
Counterstain, if desired, and mount coverslips with either a permanent mounting media or an aqueous-based mounting media. Please note, permanent mounting requires dehydration through ethanol and clearing through xylene.
Solutions and Reagents
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
限制使用
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Revision 5
