Revision 3

#79809

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-P, IF-F, FC-FP

Reactivity:
M

Sensitivity:
Endogenous

MW (kDa):
25-60

Source/Isotype:
Rabbit IgG

UniProt ID:
#P11911

Entrez-Gene Id:
12518

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:250 - 1:1000
Immunofluorescence (Frozen) 1:1600 - 1:6400
Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

CD79A (F3Z1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD79A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD79A protein.

Background

Antigen receptors found on the surface of B cells contain a heterodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B cell development and function (3). Together these two proteins and the associated B cell receptor (BCR) initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) found in the CD79A intracellular region appears to be important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). Research has shown that CD79A is a marker for B-lineage lymphoblastic leukemia (8). Additionally, investigators have found that mutations in the CD79A (MB1) gene are associated with abnormally low levels of functional B cell receptors in some cases of chronic B cell lymphocytic leukemia (9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CD79A (F3Z1H) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Negative expression of CD79A protein in C2C12 and NIH/3T3 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD79A protein (mCD79A-Myc/DDK; +), using CD79A (F3Z1H) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower).
Western Blotting Image 2: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Immunoprecipitation of CD79A protein from A20 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD79A (F3Z1H) Rabbit mAb. Western blot analysis was performed using CD79A (F3Z1H) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Immunoprecipitation Image 1: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD79A (F3Z1H) Rabbit mAb.
Immunohistochemistry Image 1: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using CD79A (F3Z1H) Rabbit mAb.
Immunohistochemistry Image 2: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse thymus using CD79A (F3Z1H) Rabbit mAb.
Immunohistochemistry Image 3: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD79A (F3Z1H) Rabbit mAb.
Immunohistochemistry Image 4: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse lung using CD79A (F3Z1H) Rabbit mAb.
Immunohistochemistry Image 5: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse liver using CD79A (F3Z1H) Rabbit mAb.
Immunohistochemistry Image 6: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse spleen (left) and colon (right) using CD79A (F3Z1H) Rabbit mAb (top) or another CD79A Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on mouse CD79a. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 7: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD79A (F3Z1H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 8: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded WEHI-231 cell pellet (left, positive) or C2C12 cell pellet (right, negative) using CD79A (F3Z1H) Rabbit mAb.
Immunohistochemistry Image 9: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse spleen using CD79A (F3Z1H) Rabbit mAb (red), CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399 (green), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse lung using CD79A (F3Z1H) Rabbit mAb (green) and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 2: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Flow cytometric analysis of fixed/permeabilized mouse splenocytes using CD79A (F3Z1H) Rabbit mAb (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left), co-stained with anti-mouse CD19 (APC Conjugate). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Flow cytometric analysis of C2C12 cells (blue, negative) and A20 cells (green, positive) using CD79A (F3Z1H) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 2: CD79A (F3Z1H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.