Revision 5

#73403

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P, IF-F, IF-IC

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
320, 250

Source/Isotype:
Rabbit IgG

UniProt ID:
#P15924

Entrez-Gene Id:
1832

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:200 - 1:800
Immunofluorescence (Frozen) 1:200 - 1:400
Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #44550.

Specificity/Sensitivity

Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total desmoplakin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human desmoplakin protein.

Background

Desmosomes are a class of intracellular junctions that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein desmoplakin, which acts as a tether by binding the cytoplasmic component of the desmosome at its N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using Desmoplakin (F3J8Y) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Negative expression of desmoplakin protein in SK-MEL-2 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged Met1-Cys1499 fragment of human Desmoplakin (hDesmoplakin (Met1-Cys1499)-Myc/DDK; +), using Desmoplakin (F3J8Y) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the salivary gland using Desmoplakin (F3J8Y) Rabbit mAb.

Immunohistochemistry Image 1: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 2: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 3: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human salivary gland using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 4: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human pancreas using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 5: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse heart using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 6: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse colon using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 7: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse ovary using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 8: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse forestomach using Desmoplakin (F3J8Y) Rabbit mAb.

Immunohistochemistry Image 9: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human heart using Desmoplakin (F3J8Y) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 10: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded HCC1187 cell pellet (left, high-expressing) or KARPAS 299 cell pellet (right, low-expressing) using Desmoplakin (F3J8Y) Rabbit mAb.
Immunohistochemistry Image 11: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse heart labeled with Desmoplakin (F3J8Y) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse colon labeled with Desmoplakin (F3J8Y) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 2: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse retina labeled with Desmoplakin (F3J8Y) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 3: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of DMS 454 cells (left, positive) and SK-MEL-2 cells (right, negative) using Desmoplakin (F3J8Y) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.