Revision 1

#70661

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
270

Source/Isotype:
Rabbit IgG

UniProt ID:
#P35749

Entrez-Gene Id:
4629

Product Usage Information

This product is the carrier free version of product #63403. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #63403

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity/Sensitivity

MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MYH11 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human MYH11 protein.

Background

MYH11 encodes the smooth muscle myosin heavy chain (SMMHC), a major specific contractile protein produced in smooth muscle (1). Mutations in MYH11 cause medial degeneration of the aorta with very low smooth muscle cell (SMC) content. Disease symptoms include aortic stiffness, aneurysm/dissection, and ductus arteriosus (2). One of the most frequent chromosomal translocations associated with acute myeloid leukemia (AML) is the fusion of CBFB, encoding the core binding protein CBFβ transcriptional factor, to MYH11 (3). This gene fusion event results in a chimeric protein that suppresses the transactivation mediated by CBF/AML-1, impairs the process of normal hematopoietic differentiation (4,5), and creates preleukemic myeloid progenitors that can be targets for AML transformation (6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged human MYH11 Asn600-Glu1972 fragment (hMYH11 (Asn600-Glu1972)-Myc/DDK; +), using MYH11 (E4N8M) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 1: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Western blot analysis of extracts from various cell lines and tissues using MYH11 (E4N8M) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Negative expression of MYH11 protein in JEG-3 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Western Blotting Image 2: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human placenta using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human prostate using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human esophagus using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human stomach using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse colon using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse lung using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 10: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse testis using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 11: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse thymus using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 12: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse prostate using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 13: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung (left), prostate carcinoma (middle), or normal breast (right) using MYH11 (E4N8M) Rabbit mAb (top) or an MYH11 Antibody (bottom). These two antibodies detect unique, non-overlapping epitopes on MYH11 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 14: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human small intestine using MYH11 (E4N8M) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).  Data were generated using the standard formulation of this product.
Immunohistochemistry Image 15: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Ri-1 cell pellet (left, positive) or JEG-3 cell pellet (right, negative) using MYH11 (E4N8M) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 16: MYH11 (E4N8M) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.