Revision 1

#60332

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Background

The synthetic peptide Z-VAD(OMe)-FMK is a cell permeable, irreversible pan-caspase inhibitor that displays non-cytotoxic effects (1-4). Z-VAD(OMe)-FMK prevents caspase-3 from adopting its active form and thereby blocks apoptosis (5). Research studies show that Z-VAD(OMe)-FMK also inhibits other enzymes, including cathepsin B, PNGase, and picornaviral 2A proteinases (6-8). Additional studies demonstrate that Z-VAD(OMe)-FMK can inhibit mitogen-induced T cell proliferation (2,9,10).

Molecular Formula

C22H30FN3O7

Molecular Weight

467.5 g/mol

Purity

>95%

CAS

187389-52-2

Solubility

Soluble in DMSO at 5mg/ml.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Directions for Use

Z-VAD(OMe)-FMK is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg of powder in 213.9 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pre-treatment at 5-100 μM for 1 hr.

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Etoposide #2200 (25 μM, 5 hr; +), either with or without Z-VAD(OMe)-FMK pretreatment (1 hr) at the indicated concentrations, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496, Caspase-8 (1C12) Mouse mAb #9746, Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625, and PARP (46D11) Rabbit mAb #9532.
undefined Image 1: Z-VAD(OMe)-FMK
Chemical structure of Z-VAD(OMe)-FMK.
undefined Image 2: Z-VAD(OMe)-FMK
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.