Revision 1

#59679

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
62

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9NP59

Entrez-Gene Id:
30061

Product Usage Information

This product is the carrier free version of product #48322. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #48322

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity/Sensitivity

SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SLC40A1/Ferroportin-1 protein. Non-specific staining was observed in pancreatic acinar cells by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of mouse tissues.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu265 of human SLC40A1/Ferroportin-1 protein.

Background

Ferroportin-1 (FPN1), also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a multi-pass membrane protein that transports iron from the inside of the cell into the blood. As the only known iron exporter, FPN1 is critical for maintaining systemic iron homeostasis (1). FPN1 activity is regulated by hepcidin, a peptide hormone secreted by the liver in response to iron loading and inflammation. Circulating hepcidin binds to FPN1 and induces its internalization and degradation, thereby reducing iron efflux to the plasma (2,3). Expression of FPN1 may also be regulated post-transcriptionally by several microRNAs that directly target its 3' untranslated region (4-6). Mutations in the SLC40A1 gene encoding FPN1 are associated with type IV hemochromatosis (7) as well as several neural tube and patterning defects, including spina bifida, exencephaly, and forebrain truncations (8). Loss of FPN1 in the brains of Alzheimer's disease (AD) mouse models and patients has been shown to promote ferroptosis, leading to neuronal death and memory impairment. Therefore, targeting of FPN1 may be a promising therapeutic approach for AD (9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of SLC40A1/Ferroportin-1 protein in REC-1 and BT-20 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Western Blotting Image 1: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Western blot analysis of extracts from human placenta and testis tissue using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of SLC40A1/Ferroportin-1 protein in human testis tissue is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Western Blotting Image 2: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SLC40A1/Ferroportin-1 protein (hSLC40A1/FPN1-Myc/DDK; +), using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 3: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human serous carcinoma of the ovary using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human placenta using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human thymus using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human lymph node using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma (left), B-cell non-Hodgkin lymphoma (middle), or normal colon (right) using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb (top) or an SLC40A1/Ferroportin-1 Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on human SLC40A1/Ferroportin-1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human spleen using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).  Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded SET-2 cell pellet (left, positive) or REC-1 cell pellet (right, negative) using SLC40A1/Ferroportin-1 (F9U5S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.