Revision 1
#4816
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H
Sensitivity:
Transfected Only
MW (kDa):
35-45
Source/Isotype:
Rabbit
UniProt ID:
#O14788
Entrez-Gene Id:
8600
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity/Sensitivity
Species predicted to react based on 100% sequence homology
Source / Purification
Background
RANKL (1), also named TNF-related activation-induced cytokine (TRANCE) (2,9), osteoprotegerin ligand (OPGL) (3), osteoclast differentiation factor (ODF) (4), and TNFSF11, is a type II transmembrane protein of the TNF family that exists as both a membrane-bound and soluble form. It is an essential regulator of immune function and bone development and homeostasis (7,10,11). RANKL is predominately expressed in activated T cells, as well as the thymus, lymph node, and bone marrow and promotes dendritic cell survival. Deletion of RANKL in mice leads to severe osteoporosis with a loss of osteoclasts, defects in T and B cell differentiation, loss of lymph node development, and mammary gland development during pregnancy (12-14).
Background References
- Anderson, D.M. et al. (1997) Nature 390, 175-9.
- Wong, B.R. et al. (1997) J. Exp. Med. 186, 2075-80.
- Lacey, D.L. et al. (1998) Cell 93, 165-76.
- Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 3597-602.
- Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-5.
- Wong, B.R. et al. (1999) Mol. Cell 4, 1041-9.
- Walsh, M.C. and Choi, Y. Cytokine Growth Factor Rev. 14, 251-63.
- Nakashima, T. et al. (2003) Curr. Opin. Rheumatol. 15, 280-7.
- Wong, B.R. et al. (1997) J Biol Chem 272, 25190-4.
- Hofbauer, L.C. (1999) Eur J Endocrinol 141, 195-210.
- Theill, L.E. et al. (2002) Annu Rev Immunol 20, 795-823.
- Mizuno, A. et al. (1998) Biochem Biophys Res Commun 247, 610-5.
- Kong, Y.Y. et al. (1999) Nature 397, 315-23.
- Fata, J.E. et al. (2000) Cell 103, 41-50.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human
Trademarks and Patents
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Revision 1