Revision 1

#47247

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
182

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q8NFA0

Entrez-Gene Id:
84669

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

USP32 (E8N8G) Rabbit mAb recognizes endogenous levels of total USP32 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu217 of human USP32 protein.

Background

Ubiquitin specific protease 32 (USP32) is a membrane-associated deubiquitinating enzyme that regulates cell proliferation and tumor metastasis (1). The USP32 gene maps to chromosomal 17q23, a region commonly amplified in breast cancer (1). Downregulation of USP32 inhibits cell proliferation, migration, and invasion in small cell lung cancer (2). In addition, expression of USP32 has been associated with tumorigenesis in gastric and ovarian cancers (3,4). One identified substrate for USP32 is the endosomal GTPase Rab7, a key regulator of endosomal trafficking. Loss of USP32 inhibits late endosome transport and recycling of late endosome cargos (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot anlaysis of extracts from various cell lines using USP32 (E8N8G) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Western Blotting Image 1: USP32 (E8N8G) Rabbit mAb
Western blot anlaysis of extracts from various cell lines using USP32 (E8N8G) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Low expression of USP32 protein in SK-BR-3 cells is consistent with the predicted expression pattern.
Western Blotting Image 2: USP32 (E8N8G) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human USP32 protein (hUSP32-Myc/DDK; +), using USP32 (E8N8G) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Western Blotting Image 3: USP32 (E8N8G) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunoprecipitation of USP32 protein from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is USP32 (E8N8G) Rabbit mAb. Western blot analysis was performed using USP32 (E8N8G) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: USP32 (E8N8G) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.