Revision 1
#4573
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-IC
Reactivity:
H M
Sensitivity:
Endogenous
MW (kDa):
87
Source/Isotype:
Rabbit
UniProt ID:
#Q15052
Entrez-Gene Id:
9459
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
Specificity/Sensitivity
Species predicted to react based on 100% sequence homology
Source / Purification
Background
X-chromosomal genes mutated in nonspecific mental retardation (MRX) comprise a family of genes, including the gene encoding Cool2/αPix, thought to be involved in mental retardation (3,4).
Cool2/αPix interacts with β-parvin/affixin, a protein involved in integrin signaling (5), and may act downstream of integrin-linked kinase (ILK) to regulate actin reorganization and cell spreading (6).
When Cool2αPix exists as a dimer, it functions as a Rac-specific GEF, whereas the monomeric protein acts as a GEF for both Rac and Cdc42. Regulation of Cool2/αPix dimerization, and therefore its specificity, occurs at least in part through p21 activated kinase (PAK) in response to extracellular signaling (7). Further, binding of Cdc42 enhances the Rac GEF activity of the Cool2/αPix dimer. Activated Rac in turn inhibits Cool2/αPix Rac GEF activity (8).
Background References
- Koh, C.G. et al. (2001) J Cell Sci 114, 4239-51.
- Kutsche, K. and Gal, A. (2001) Cytogenet Cell Genet 95, 196-201.
- Kutsche, K. et al. (2000) Nat Genet 26, 247-50.
- Lower, K.M. and Gecz, J. (2001) Am J Med Genet 100, 43-8.
- Rosenberger, G. et al. (2003) Hum Mol Genet 12, 155-67.
- Filipenko, N.R. et al. (2005) Oncogene 24, 5837-49.
- Feng, Q. et al. (2004) EMBO J 23, 3492-504.
- Baird, D. et al. (2005) Curr Biol 15, 1-10.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human M: Mouse
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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Revision 1
