Revision 1

#44498

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IF-F

Reactivity:
M R

Sensitivity:
Endogenous

MW (kDa):
57

Source/Isotype:
Rabbit

UniProt ID:
#Q9H598

Entrez-Gene Id:
140679

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Frozen) 1:50 - 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

VGAT Antibody recognizes endogenous levels of total VGAT protein. Non-specific labeling in the crypts of small intestine is observed by immunofluorescence in fixed frozen mouse tissue. By western blot, this antibody detects a band of unknown origin at approximately 40 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VGAT protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The vesicular GABA transporter (VGAT), also known as the vesicular inhibitory amino acid transporter (VIAAT), loads GABA and glycine from the neuronal cytoplasm into synaptic vesicles for vesicular storage and subsequent exocytosis of these inhibitory amino acids. VGAT is present in both GABAergic and glycinergic neurons, with its expression limited to the brain (1,2). It can also recognize β-alanine as a substrate (2).

Recently, tau accumulation, as found in Alzheimer’s disease (AD) patients, has been shown to selectively suppress the expression of the intracellular VGAT (3). At the same time, VGAT activation can help with anxiety exacerbated by AD pathology (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from mouse lung and brain tissue using VGAT antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of VGAT protein in mouse lung tissue is consistent with the predicted expression pattern.
Western Blotting Image 1: VGAT Antibody
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus, labeled with VGAT Antibody (left, green) and co-labeled with TMEM119 (E4B9S) Mouse mAb #98778 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 1: VGAT Antibody
Confocal immunofluorescent analysis of fixed frozen mouse retina labeled with VGAT Antibody (green) and co-labeled with ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 2: VGAT Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse spinal cord, labeled with VGAT Antibody (left, green) and co-labeled with TMEM119 (E4B9S) Mouse mAb #98778 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 3: VGAT Antibody
Confocal immunofluorescent analysis of fixed frozen mouse striatum, labeled with VGAT Antibody (left, green) and co-labeled with TMEM119 (E4B9S) Mouse mAb #98778 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 4: VGAT Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.