Revision 10

#38428

Store at +4C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IHC-Bond, IHC-P

Reactivity:
H

Sensitivity:
Endogenous

Source/Isotype:
Mouse IgG1

UniProt ID:
#P16150

Entrez-Gene Id:
6693

Product Usage Information

Application Dilution
IHC Leica Bond 1:50 - 1:200
Immunohistochemistry (Paraffin) 1:50

Storage

Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.

Specificity/Sensitivity

CD43 (MT1) Mouse Monoclonal Antibody recognizes endogenous levels of CD43 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human lymphocytes.

Background

CD43, also known as leukosialin and sialophorin, is a type I transmembrane sialylated, negatively charged mucin that is expressed on the surface of most hematopoietic cells (1,2). CD43 plays an important role in T lymphocyte function by negatively regulating antigen-specific T cell activation and interfering with cellular adhesion and proliferation (2,3). It also has been reported that CD43 is involved in viral clearance (3). Aberrant expression of CD43 is associated with several diseases, such as Wiskott-Aldrich syndrome and acute leukemia (3,4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD43 (MT1) Mouse mAb performed on the Leica BOND Rx.
Immunohistochemistry Image 1: CD43 (MT1) Mouse Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD43 (MT1) Mouse mAb performed on the Leica BOND Rx.
Immunohistochemistry Image 2: CD43 (MT1) Mouse Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human Hodgkin lymphoma using CD43 (MT1) Mouse mAb performed on the Leica BOND Rx.
Immunohistochemistry Image 3: CD43 (MT1) Mouse Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD43 (MT1) Mouse mAb.
Immunohistochemistry Image 1: CD43 (MT1) Mouse Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human small cell carcinoma of the salivary gland using CD43 (MT1) Mouse mAb.
Immunohistochemistry Image 2: CD43 (MT1) Mouse Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using CD43 (MT1) Mouse mAb.
Immunohistochemistry Image 3: CD43 (MT1) Mouse Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using CD43 (MT1) Mouse mAb.
Immunohistochemistry Image 4: CD43 (MT1) Mouse Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human esophagus using CD43 (MT1) Mouse mAb.
Immunohistochemistry Image 5: CD43 (MT1) Mouse Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human small intestine using CD43 (MT1) Mouse mAb.
Immunohistochemistry Image 6: CD43 (MT1) Mouse Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human appendix using CD43 (MT1) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right).
Immunohistochemistry Image 7: CD43 (MT1) Mouse Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded HL-60 cell pellet (left, positive) or VCaP cell pellet (right, negative) using CD43 (MT1) Mouse mAb.
Immunohistochemistry Image 8: CD43 (MT1) Mouse Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.