Revision 2

#37188

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

MW (kDa):
22.5

UniProt ID:
#P55075, #P37237

Entrez-Gene Id:
2253, 14179

Background

FGF-8 is a member of the large and diverse fibroblast growth factor (FGF) family of peptide growth factors. FGF-8 binds to receptor FGFR1 and functions as a signaling molecule that is involved in many important processes (1,2). FGF-8 is involved in the development of the forebrain and also plays a role in maxillofacial diseases (3,4). It regulates craniofacial structure formation including the teeth, palate, mandible, and salivary glands and decreased expression of FGF-8 results in malformations such as absence of molar teeth, failure to close the palate, and decreased mandible size (4).

Endotoxin

Endotoxin levels are less than or equal to 1 EU / 1 μg h/mFGF-8.

Purity

A greater than or equal to 95% purity was determined by SDS-PAGE.

Source / Purification

Recombinant human/mouse FGF-8 was expressed in E. coli and is supplied in a lyophilized form.

Bioactivity

The bioactivity of recombinant h/mFGF-8 was determined in an NR6R-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 150 ng/mL.

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Serial dilutions of Human/Mouse FGF-8 Recombinant Protein were added to NR6R-3T3 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
undefined Image 1: Human/Mouse FGF-8 Recombinant Protein
The purity of Human/Mouse FGF-8 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant h/mFGF-8 and staining with Coomassie Blue.
undefined Image 2: Human/Mouse FGF-8 Recombinant Protein
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.