Revision 1

#33750

Store at -20C

ZAP (E5V4D) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
80, 100

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q7Z2W4

Entrez-Gene Id:
56829

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

ZAP (E5V4D) Rabbit mAb recognizes endogenous levels of total ZAP protein. This antibody recognizes ZAP isoforms 1 and 2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp210 of human ZAP protein.

Background

ZAP (Zinc finger CCCH-type antiviral protein 1, ZC3HAV1, PARP13) is an RNA-binding protein which inhibits the replication of certain viruses, including HIV-1, by preventing the accumulation of viral RNA in the cytoplasm (1-5). ZAP binds to specific viral mRNAs through the CCCH zinc finger motifs located on its N terminus, and recruits exosomes and decapping enzymes to degrade the target RNA (3-5). ZAP also interferes with the interaction between eIF4A and eIF4G on target mRNA, causing translational repression (6). Isoform 2, the shorter isoform also known as ZAPS, acts as a stimulator of interferon responses in human cells mediated by the RNA helicase RIG-I as part of the innate antiviral immune response (7).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#33750

ZAP (E5V4D) Rabbit mAb

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using ZAP (E5V4D) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of ZAP protein in SJCRH30 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: ZAP (E5V4D) Rabbit mAb
Western blot analysis of extracts from various cell lines using ZAP (E5V4D) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: ZAP (E5V4D) Rabbit mAb
Western blot analysis of extracts from FaDu cells, transfected with control siRNA (-) or human ZAP siRNA (hZAP siRNA; +), using ZAP (E5V4D) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 3: ZAP (E5V4D) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.