| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody | 4228 | 20 µl | 60 and 85 kDa | Rabbit |
| PI3 Kinase p85 (19H8) Rabbit mAb | 4257 | 20 µl | 85 kDa | Rabbit IgG |
| PI3 Kinase p110α (C73F8) Rabbit mAb | 4249 | 20 µl | 110 kDa | Rabbit IgG |
| PI3 Kinase p110β (C33D4) Rabbit mAb | 3011 | 20 µl | 110 kDa | Rabbit IgG |
| PI3 Kinase Class III (D4E2) Rabbit mAb | 3358 | 20 µl | 100 kDa | Rabbit IgG |
| PI3 Kinase p110γ (D55D5) Rabbit mAb | 5405 | 20 µl | 110 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The PI3 Kinase Sampler Kit provides an economical means of studying PI3 kinase subunits in cells. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.
Storage
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).
Background References
Trademarks and Patents
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