Revision 5

#4697Store at -20C

10 µg

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not For Use In Diagnostic Procedures.
MW (kDa):


UniProt ID:


Entrez-Gene Id:



Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs are synthesized as precursor proteins that are processed by cleavage to produce mature proteins. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting in the translocation of Smad to the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).
  1. Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
  2. Wozney, J.M. et al. (1988) Science 242, 1528-34.
  3. Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
  4. Nohe, A. et al. (2004) Cell Signal 16, 291-9.




Source / Purification

Recombinant human BMP-2 was expressed in E. coli and is supplied in a lyophilized form.


The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml.

Product Usage Information

¶The working concentration of recombinant human BMP-2 generally ranges from 20-50 ng/ml.


Lyophilized product is very stable at -20°C. It is recommended to reconstitute with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Background References

    Revision 5

    Human BMP-2 Recombinant Protein

    Human BMP-2 Recombinant Protein: Image 1
    ATDC-5 cells were cultured with 0 to 1000 ng/mL of recombinant hBMP-2. Alkaline phosphatase activity was measured after 67 hours by measuring the OD405.
    Human BMP-2 Recombinant Protein: Image 2
    The purity of recombinant hBMP-2 was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hBMP-2 and staining overnight with Coomassie Blue.
    Human BMP-2 Recombinant Protein: Image 3
    Western blot analysis of extracts from serum-starved HT-1080 cells, untreated and treated for 30 minutes with various concentrations of recombinant hBMP-2, using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 (upper) and Smad1 Antibody #9743 (lower).
    Revision 5


    Human BMP-2 Recombinant Protein


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