Revision 6

#18350Store at -20C

1 Kit

(6 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb 44463 20 µl 110-130, 125-140, 180-200 kDa Rabbit IgG
Phospho-Axl (Tyr702) (D12B2) Rabbit mAb 5724 20 µl 138 kDa Rabbit IgG
Axl (C89E7) Rabbit mAb 8661 20 µl 138 kDa Rabbit IgG
Mer (D21F11) XP® Rabbit mAb 4319 20 µl 210 kDa Rabbit IgG
Tyro3 (D38C6) Rabbit mAb 5585 20 µl 110, 130 kDa Rabbit IgG
GAS6 (D3A3G) Rabbit mAb 67202 20 µl 78 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Tyro/Axl/Mer Activation Antibody Sampler Kit provides an economical means of detecting the activation of TAM family members using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Axl, Mer and Tyro3 are three members of the TAM family receptor tyrosine kinase that share a common NCAM (neural adhesion molecule)-related extracellular domain and a conserved intracellular tyrosine kinase domain. These receptors bind common homologous vitamin K dependent protein GAS6 and protein S to activate downstream signaling pathways (1). TAM family receptors are involved in the development of immune, nervous, vascular and reproductive systems, autoimmune disease, cancer drug resistance and tumor immunity response (2-5). Axl (Tyr698), Axl (Tyr702), Mer Tyr(749) and Tyro3 (Tyr681) are conserved autophosphorylation sites located in the activation loop of the respective tyrosine kinase domains. Phosphorylation at these sites is required for full kinase activation of each of the corresponding receptors (6,7).

  1. Rothlin, C.V. et al. (2015) Annu Rev Immunol 33, 355-91.
  2. Burstyn-Cohen, T. (2017) Int J Dev Biol 61, 215-24.
  3. Bellan, M. et al. (2016) Int J Mol Sci 17, pii: E1807.
  4. Schoumacher, M. and Burbridge, M. (2017) Curr Oncol Rep 19, 19.
  5. Akalu, Y.T. et al. (2017) Immunol Rev 276, 165-77.
  6. Ling, L. et al. (1996) J Biol Chem 271, 18355-62.
  7. Onken, J. et al. (2017) Oncotarget 8, 50403-14.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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